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KMID : 0618720090150010077
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Journal of The Korea Institute of Oriental Medicine Informatics
2009 Volume.15 No. 1 p.77 ~ p.84
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The Effect of seven Korean Traditional Herbal Medicines on 3T3-L1 Adipocyte Differentiation
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Kim Ji-Seong
Cho Ga-Young
Kim Eun-Joo
Park Jun-Seong
Rho Ho-Sik
Park Hye-Yoon
Kim Duck-Hee
Kim Han-Gon
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Abstract
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Objectives : The aim of the present study is to evaluate the effects Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae extracton 3T3-L1 preadipocytes differentiation.
Methods : We adopted Oil Red O staining methods to observe the formed lipid droplets. And the amount of lipids in adipocytes was measured using absorptiometric analysis.
Results : The extracts of Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae stimulated the preadipocytes differentiation and lipid droplet formation. And the complex extract of the traditional herbal medicines stimulated there actions more than single extracts.
Conclusions : The extract of Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae has stimulatory effects. on adipogenesis. Moreover, the complex extract of the traditional herbal medicines has more effect than single extracts.
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KEYWORD
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Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, Radix glycyrrhizae, 3T3-L1 preadipocytes, differentiation, Oil Red O staining
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